Limitations of gel filtration for serum unbound calcium and magnesium
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چکیده
منابع مشابه
Limitations of gel filtration for serum unbound calcium and magnesium.
Serum calcium exists in three forms: pr,,tein-bound, free ionic, and complexed in a small molecule t. Ionic and protein-bound forms interact to produce an equilibrium influenced by pH, protein concentration, and to a lesser degree by temperature and other factors t. Free ions and complexes are identified as 'unbound, diffusible, ultrafilterable' species. Serum magnesium is believed to have simi...
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نتایج این مطالعه نشان می دهد که مقدار کلسیم (یونیزه و تام)، منیزیم و فسفر به طور معنی داری تغییر پیدا کرد به جزء مقدار کلسیم تام و منیزیم در روش داخل عضلانی که تغییر معنی داری نداشته است. بر اساس نتایج این مطالعه می توان نتیجه گیری کرد که اکسی تتراساکلین بر روی مقادیر سرمی کلسیم و منیزیم تأثیر می گذارد باید مقدار این کاتیون ها در بیماران، در هنگامی که اکسی تتراسایکلین استفاده می شود مورد توجه ق...
15 صفحه اولSeparation and quantitation of serum constituents associated with calcium by gel filtration.
Gel filtration of serum by use of polyacrylamide beads (Biogel P-2) separates total colcium into four distinct peaks: an initial peak, corresponding to protein-bound calcium; a second peak containing the calcium complexes of citrate, phosphate, lactate, and sulfate; a third peak containing calcium bicarbonate; and a fourth peak or trough corresponding to the serum ionic calcium. An eluent conta...
متن کاملA micro-method for serum calcium and serum magnesium.
A simple, rapid, accurate method for determining calcium and magnesium separately in 0.1 ml. volumes of serum (or heparinized plasma) was required for the routine chemical pathology service of a children's hospital (Wilkinson, 1955-6). The apparatus used had to be generally available, the minimum of pipetting and transfer was desirable for the sake of accuracy, and the method had to be specific...
متن کاملUse of gel filtration to examine the distribution of calcium among serum proteins.
Gel filtration of serum by use of dextran bisacrylamide beads ("Sephacryl S-200," fractionation range: 5000-250 000 daltons), separates the serum proteins into three distinct peaks. We used an eluent containing (per liter) 140 mmol of sodium, 1.10 mmol of calcium, 0.50 mmol of magnesium, and 10 mmol of 2-([2-hydroxy-1, 1-bis(hydroxymethyl)ethyl]amino)ethanesulfonate buffer (pH 7.43 at 37 degree...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 1970
ISSN: 0009-8981
DOI: 10.1016/0009-8981(70)90369-4